The DNA Analysis Blog

Practical guides on primer design, sequence analysis, restriction mapping, and more. Written for researchers and students.

🧪 5'-ATGCGATCGATCG-3' 3'-TACGCTAGCTAGC-5'
Primer Design
How to Design PCR Primers: A Step-by-Step Guide
Learn how to pick primers with the right melting temperature, GC content, and length for reliable PCR amplification.
7 min readPrimer Design
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GC Content (%)
Sequence Analysis
What is GC Content and Why Does It Matter?
GC content affects melting temperature, secondary structure, and primer binding. Here is what you need to know.
6 min readGC Content
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6 Reading Frames ATG...TAA ATG...TGA
ORF Finder
How to Find Open Reading Frames in a DNA Sequence
Open reading frames reveal where genes are encoded. This guide explains how to identify ORFs across all 6 reading frames.
7 min readORF Analysis
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EcoRI BamHI HindIII Linear Restriction Map
Restriction Mapping
Restriction Enzyme Mapping Explained
Restriction enzyme maps show where specific enzymes cut your DNA. Learn how to read and generate them for cloning and analysis.
6 min readCloning
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ATG GGC AAA CTG TTC M G K L F CAI Score: 0.87
Codon Optimization
Codon Optimization for Protein Expression
Rare codons slow down ribosomes and reduce protein yield. Learn how codon optimization boosts expression in your host organism.
8 min readProtein Expression
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CpG Island CpG Methylation and Gene Silencing
Epigenetics
CpG Islands and Gene Regulation
CpG islands are found near gene promoters and play a key role in methylation and gene silencing. Learn how to detect and interpret them.
7 min readEpigenetics
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