Everything You Need for
Sequence Analysis
A complete bioinformatics suite built for researchers, students, and professionals. No installation, no subscription.
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Primer Design
SantaLucia nearest-neighbor thermodynamics for accurate Tm. Hairpin/dimer ฮG, 3โฒ stability, GC clamp, poly-run detection, and heterodimer checking.
SantaLucia NNHairpin ฮGHeterodimer
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Restriction Enzyme Mapping
Visual linear restriction map with canvas-based ruler, backbone, staggered cut markers, per-enzyme lanes, and an interactive legend.
Visual Map50+ EnzymesCanvas
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Codon Optimization
CAI-weighted RSCU engine with rare codon flagging, per-codon frequency table, and CAI score display for multiple host organisms.
CAI ScoreRSCURare Codons
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ORF Finder
Detect open reading frames across all 6 reading frames. Translates to amino acid sequences with codon-by-codon highlighting.
6 FramesTranslationHighlighting
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CpG Island Detection
Identifies CpG islands using observed/expected ratio and GC content thresholds. Visualized with a color-coded map overlay.
Obs/Exp RatioVisual Map
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RNA Secondary Structure
Predicts RNA hairpin structures using free energy minimization. Displays stem-loop diagrams with ฮG values.
HairpinฮGStem-Loop
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GC Content Analysis
Window-based GC% sliding analysis with interactive chart. Highlights AT-rich and GC-rich regions across the full sequence.
Sliding WindowChart
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Repeat Finder
Detects tandem repeats, inverted repeats, and direct repeats. Annotates positions and repeat unit lengths across the sequence.
TandemInvertedDirect
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Kozak Scorer & AA Properties
Scores Kozak consensus strength for translation initiation. Amino acid property analysis including hydrophobicity, charge, and molecular weight.
KozakHydrophobicityMW